DTlite Real Time PCR Thermal Cycler

DTlite Real Time PCR Thermal Cycler

DTlite is innovational solutions in a compact body.

Advantages of DTlite:
high performance thanks to a wide range of thermal blocks in 48 and 192 wells format adapted to microplates, tubes and strips;
up to 5-channel multiplex fluorescence detection for a variety of fluorescence dyes and fluophlore combinations in a wide spectral range. The standard set of DTlite is supplied with 4 channels set for detection of Hex, Fam, Cy5, Rox dyes. (It is possible to install a filter for fluorescence detection in the range of Cy5,5);
light emitting diodes (LED) as a light source that, unlike halogen lamps, does not deteriorate over the life-time of about 100,000 hours;
high sensitivity, optimal signal/noise ratio and a low interchannel crosstalk ensured by the unique design of the optical track including a separate light source for each channel and a matrix CCD camera;
broad dynamic range of detection achieved by employing multiple exposure method, which takes the optimization of signal registration conditions to a whole new level, greatly simplifying or even eliminating the need for photometric settings;
optical systems with various spectrum parameters enable operation with multiple fluorophlores;
the thermal block makes it possible to set the gradient of annealing and/or thermal block temperature difference, which opens additional possibilities for optimizing amplification programs;
keeping regular thermal block temperature, which provides for high reliability and replication of results;
the device has protection from interruptions of network power supply (automatic resuming of amplification program following the restoration of network power supply);
the device has protection from computer failures (automatic resuming of device operation in case of any problems with the computer (up to its replacement) with automatic reading of results of optical measurements from the device to computer following their elimination);
realized is the possibility of operator’s diagnostics of the thermal cycling system and the device optical system directly in the process of amplification program running;
provided for is the possibility of work with the program both in the compact and extended modes, which provides for comfortable environment for clinical and research applications;
the possibility of work with robotized sample preparation systems thanks to special design of the body and automatically-operated draw-out thermal block;
several devices can be controlled simultaneously by one computer. Moreover any batch of data can be viewed and analyzed by the user using computer while another amplification program is being executed;
the compact body design, allowing several devices to be placed closer together to increase productivity at minimal space requirements.

Technical Parameters of DTlite detecting amplifiers of L and S modification

Parameter Value
Design of the thermal plate

2-sectional module for 48 test tubes 0.2 μL with possibility of adjusting the temperature difference between sections (8×6 format, modification S1);
one piece unit (non-separated unit) for 192 test tubes 0.045 ml (16×12 format, modification L1)

Tube type for S modification devices:
— standard microtubes 0.2 ml for real-time PCR
— strips 8×0.2 ml for real-time PCR
for L modification devices:
— microplate 192×0.045 ml (half of a standard microplate 384×0.045 ml) for real-time PCR
Maximum volume of reaction mix 0.1 ml (for S modification devices)
0.03 ml (for L modification devices
Heating ramping rate, average (maximal) 5 °С / sec (for S modification devices)
3.5 °С / sec (for L modification devices)
Cooling ramping rate, average (maximal) 2.5 °С / sec (for S modification devices)
1.5 °С / sec (for L modification devices)
Temperature range of the thermal unit 0°… 100 °С
Temperature setting increment 0.1°C
Precision of temperature maintenance ±0.2 °С
Temperature irregularity of thermal unit ±0.3°С
Thermal block lid heating (maximal) 105 °C
Possibility of simultaneous detection of amplification products (detection channels) 4 (modification DTlite 4)
5 (modification DTlite 5)
Spectral range 470 nm — 675 nm for devices with 4 detection channels (DTlite 4)
470 nm — 750 nm for devices with 5 detection channels (DTlite 5)
Power supply network voltage 220 V
Power supply network frequency 50 Hz
Dimensions (LxWxH) 210 x 480 x 310 mm
Weight 17 kg

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