PCR test systems
AgroDiagnostica, LLC has developed and implemented into practice a unique set of test systems based on polymerase chain reaction (PCR) for diagnosis and identification of a wide range of crop infectious diseases. Also developed were systems for nucleic acid isolation from plant material and preparation of samples for PCR analysis. Kits are manufactured in three different formats: analysis of PCR products by electrophoresis (E), analysis based on FLASH (FLuorescent Amplification-based Specific Hybridization) technology, and Real-Time PCR (Rt). With FLASH technology, fluorescence is registered with a PCR detector “Gene" or “Gene 4” ("DNA Technology" Research and Production Company, Russia), the results are displayed in 30-60 seconds. Sets based on FLASH technology do not require opening the tubes after amplification, which significantly reduces the risk of contamination. Reagent kits in the Rt format allow qualitative analysis and are adapted for such devices as DTprime, DTlite (DNA Technology, Russia), iQ5 iCycler (Bio-Rad Laboratories, USA). We also have kits adapted to Rotor-Gene 6000 (Qiagen Instruments, Germany).
The developed systems allow detecting and identifying pathogens very quickly, reliably and with high sensitivity.
| Description | Detection formats | ||
| FLASH | Rt | ||
| Kits of reagents for detection of potatoes’ pathogens | |||
| Clavibacter michiganensis subsp. Sepedonicus |
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| Ralstonia solanacearum |
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| Potato Leafroll Virus |
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| Potato Virus M |
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| Potato Virus S |
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| Potato Virus X |
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| Potato Spindle Tuber Viroid |
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| Potato Virus Y |
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| Potato Virus A |
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| Potato Mop Top virus |
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| Andean potato mottle virus |
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| Andean potato latent virus |
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| Globodera pallida |
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| Globodera rostochiensis |
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| Synchytrium endobioticum |
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| Potato black ringspot virus |
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| Potato virus T |
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| Potato yellowing virus |
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| Potato yellow dwarf virus |
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| Kit of reagents for amplification of potato actin gene cDNA |
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| Kits of reagents for detection of sugar beets’ pathogens | |||
| Beet necrotic yellow vein virus |
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| Pseudomonas syringae |
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| Kits of reagents for detection of pathogens of cucumbers and tomatoes grown indoor | |||
| Xanthomonas euvesicatoria |
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| Pseudomonas corrugata |
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| Pseudomonas syringae pv. Lacrimans |
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| Clavibacter michiganensis subsp. michiganensis |
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| Pectobacterium carotovorum subsp. сarotovorum |
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Ascochyta cucumis; Phoma cucurbitacearum; Didymella bryoniae
Ascochyta lycopersici Didymella, Phoma |
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| Tomato ringspot virus |
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| Tomato yellow leaf curl begomovirus |
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| Tomato spotted wilt virus |
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| Tomato brown rugose fruit virus |
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Kits of reagents for detection of cereals’ pathogens Septoria sp. |
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| Septoria nodorum |
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| Septoria tritici |
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Kits of reagents for detection of cereals’ pathogens Fusarium sp. |
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| Fusarium graminearum |
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| Fusarium culmorum |
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| Fusarium sporotrichioides |
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| Fusarium langsethiae |
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| Fusarium poae |
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| Fusarium avenaceum |
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| Fusarium tricinctum |
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| Fusarium cerealis |
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| Fusarium acuminatum* |
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| Fusarium torulosum* |
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| Fusarium solani* |
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| Kits of reagents for the detection of Fusarium fungi with similar profiles of the produced toxins | |||
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Toxins: DON, 3-ADON, 15-ADON, NIV, FUSX, ZEA Primary producers: Fusarium graminearum, Fusarium culmorum, Fusarium cerealis |
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Toxins: T-2, HT-2, DAS, NEO, ZEA Primary producers: Fusarium sporotrichiodes, Fusarium langsethiae |
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Toxins: NIV, FUSX, T-2, HT-2, DAS, ZEA Primary producers: Fusarium poae |
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Toxins: MON, BEA, ENN A, B Primary producers: Fusarium avenaceum, Fusarium tricinctum, Fusarium acuminatum, Fusarium torulosum |
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| Kits of reagents for diagnosis of pathogens and pests of fruit crops | |||
| Plum Pox virus |
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| Erwinia amilovora |
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| Ceratitis capitata |
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| Raspberry ringspot virus |
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| Candidatus Phytoplasma Vitis |
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| Candidatus Phytoplasma pyri |
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| Candidatus Phytoplasma mali |
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| Peach latent mosaic viroid |
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| Peach rosette mosaic virus |
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| Kits of reagents for detection of quarantine’ pathogens | |||
| Tomato leaf curl New Delhi virus, ToLCNDV New! |
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| Pepino mosaic virus New! |
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| Clavibacter michiganensis subsp. Sepedonicus |
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| Erwinia amilovora |
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| Ralstonia solanacearum |
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| Plum Pox virus |
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| Globodera pallida |
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| Globodera rostochiensis |
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| Bursaphelenchus xylophilus |
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| Bursaphelenchus mucronatus |
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| Pantoea stewartii |
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| Ceratitis capitata |
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| Beet necrotic yellow vein virus |
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| Andean potato mottle virus |
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| Andean potato latent virus |
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| Phomopsis helianthi |
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| Synchytrium endobioticum |
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| Potato black ringspot virus |
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| Raspberry ringspot virus |
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| Tobacco ringspot virus |
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| Tomato ringspot virus |
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| Candidatus Phytoplasma Vitis |
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| Candidatus Phytoplasma pyri |
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| Candidatus Phytoplasma mali |
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| Peach latent mosaic viroid |
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| Tomato yellow leaf curl begomovirus |
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| Tomato spotted wilt virus |
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| Acidovorax citrulli |
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| Impatiens necrotic spot tospovirus |
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| Potato virus T |
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| Potato yellowing virus |
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| Chrysanthemum stem necrosis virus |
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| Chrysanthemum stunt viroid |
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| Potato yellow dwarf virus |
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| Xanthomonas oryzae pv. Oryzae |
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| Xanthomonas campestris pv. Campestris |
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| Peach rosette mosaic virus |
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| Tomato brown rugose fruit virus |
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| Barley stripe mosaic virus, BSMV |
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| Wheat streak mosaic virus, WSMV |
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| Brome mosaic virus, BMV |
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| Maize dwarf mosaic virus, MDMV |
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| DNA/RNA extraction Kits | |||
| PREP-NA (DNA/RNA extraction) | |||
| PREP-GS | |||
| PREP-TsTAB | |||
| Reverse transcriptase | |||
| PCR Sets | |||
| Basic PCR Sets |
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417 Kb
14 Mb
1,3 Mb
